Chelsea, Real Madrid, Bayern Munich, and Paris Saint-Germain players observed minutes of silence in memory of Diogo Jota.
The clubs are in the United States, competing in the Club World Cup.
Several of the players who observed the minute silence are Jota’s former club and international teammates.
PSG’s tribute was especially poignant given the presence of four Portuguese players in their team.
The Portugal forward and his brother Andre Silva died in an accident in the Spanish province of Zamora.
His 26-year-old brother was also a professional footballer with Portuguese second-tier club Penafiel.
The Guardia Civil has told BBC Sport that Jota and his brother died at about 00:30 local time on Thursday.
It was reported that their car, a Lamborghini, left the road due to a tyre blowout while overtaking another vehicle and then caught fire.
Jota married his long-term partner, Rute Cardoso, with whom he had three children, just last month.
Jota helped Liverpool win the Premier League title last season and also played for Portugal as they beat Spain in the final of the Nations League in June.
“The Portuguese Football Federation (FPF) and all of Portuguese football are completely devastated,” read an FPF statement.
“Much more than an amazing player, with almost 50 international appearances, Diogo Jota was an extraordinary person, respected by all team-mates and opponents, someone with an infectious joy and a reference in the community itself.
“We have lost two champions. The passing of Diogo and Andre Silva represents irreparable losses for Portuguese football, and we will do everything to honour their legacy daily.”
The FPF has requested that European football’s governing body UEFA observe a minute’s silence before Portugal’s Women’s Euros game against Spain on Thursday.
Jota spent the 2016-17 season on loan with Porto, where his brother was a youth player.
“It is with shock and deep sorrow that we send our heartfelt condolences to the family and friends of Diogo Jota and his brother Andre Silva,” read a club statement. “Rest in peace.”
